Skip Navigation

History

As the common marmoset has become a major primate model species in behavioral neuroscience, including immunocytochemistry, MRI and neuroendocrinology, the need to have reliable neuroanatomical images to assist in identifying brain structures of interest has become increasingly important. A printed stereotaxic brain atlas by Stephan, et al. has been out of print for some time. Hence, my lab made the commitment to prepare a new brain atlas and make it available on-line. Six adult marmosets, 3 males and 3 females, were selected for this project. Each was deeply anesthetized with pentobarbital sodium and the brain fixed by intracardiac perfusion of PBS followed by 10% formalin. The brains were carefully dissected and kept in formalin until delivered to Neuroscience Associates in Knoxville, TN, who prepared the brains under the direction of Dr. Robert Switzer. After embedding, one brain of each sex was cut at 40 microns in the coronal, sagittal and horizontal planes. Every sixth section was stained with thionine for Nissl granules and every seventh section stained for myelin fibers with a modified Weil technique. The brain atlas presented here was prepared from the coronal sections of the female brain. The AP 0 section was chosen after perusal of the MRI images; the "section" with the ear canals clearly in view was chosen as the template for selecting the AP 0 from our brain. It is a different section than the one illustrated as "AP 0" in the Stephan atlas. This may be due to a different angle of sectioning for the 2 brains, the use of a male brain in the Stephan atlas, or some other factor. Since the present atlas is not intended for stereotaxic use, the "A" or "P" labels on our sections are mainly for referencing the sections containing a specific structure.